HEPES, Free Acid
HEPES, Free Acid
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look up the pka of hepes and determine what would be the most efficient way to make up a hepes buffer at ph 85 list what starting compounds and reagents you would use 42313
look up the pka of hepes and determine what would be the mos…
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A purified protein is in a HEPES (N-(2-hydroxyethyl)piperazine-N-(2-ethanesulfonic acid)) buffer at pH 7 with 800 mM NaCl. A 1 mL sample of the protein solution is placed in a tube made of dialysis membrane and dialyzed against 2.0 L of the same HEPES buffer with 0 mM NaCl. Small molecules and ions, such as Na, Cl, and HEPES, can diffuse across the dialysis membrane, but the protein cannot.Once the dialysis has come to equilibrium, what is the concentration of NaCl in the protein sample? Assume
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