Most sporadic solid tumors require several mutational genetic events prior to acquisition of a malignant phenotype and development of neoplasia. Age-specific incidence data in head and neck squamous ...

The relative levels of the deoxycytidine kinase (dCK), deoxyguanosine kinase (dGK), and the 5′-nucleotidase (5′-NT) are of importance for the effect of many nucleoside analogues used in the treatment ...

The polymerase chain reaction (PCR), invented about three decades ago, soon entered mainstream use thanks to an ongoing series of refinements. One particularly important refinement, introduced about ...

Real-time PCR or quantitative PCR (Polymerase Chain Reaction) is an advancement of the standard PCR technique, which enables continuous monitoring of the reaction and collection of data in real time.

What is real-time quantitative PCR? qPCR uses one of two methods to detect the changes to DNA amplification: use of fluorescent dyes or sequence-specific DNA probes. qPCR is normally performed in a ...

In recent years, quantitative real-time PCR tests have been extensively developed in clinical microbiology laboratories for routine diagnosis of infectious diseases, particularly bacterial diseases.

This infographic highlights the principles, strengths and limitations of qPCR while showcasing its applications across fields ...

The real-time polymerase chain reaction( PCR) has become a key tool for molecular assay development, expanding into applications as diverse as in vitro diagnostics (IVDs), food safety testing, and ...

The evolution of PCR technology is shaping the future of diagnostics, especially in viral detection and RNA analysis. In the second episode of our New Frontiers in PCR Technology Series, we have ...

A quantitative, real-time, nested polymerase chain reaction (PCR) method, combining the high sensitivity of nested PCR with time-saving real-time instrumentation, was developed for large-scale ...

The biolistic method is reliable for delivering genes of interest into various species, but low transformation efficiency can be a limiting factor in its application. To test various conditions that ...

Young Adults With Acute Lymphoblastic Leukemia Have an Excellent Outcome With Chemotherapy Alone and Benefit From Intensive Postinduction Treatment: A Report From the Children's Oncology Group ...